State Lab Report

State Health Institute

Laboratories of the Center of Epidemiology and Microbiology National Reference Laboratory for Disinfection and Sterilization

Šrobárova 49/48
100 00 Praha 10
tel. 26708 2284

Determination of TiO2 composite efficiency applied to Al material on the liquidation of the virus and irradiation of the UV lamp according to ISO 22196 and JIS Z 2801 methods modified to bacteriophagus

Laboratory expertise Nr. 200355/2020

Execution date of the expertise report: April 20, 2020

Anti-virus effect (including virucidal activity)

MUDr. Věra Melicherčíková, CSc.
Ing. Jan Urban, Ph.D.
Mgr. Kateřina Opravilová

Technical support cooperation: Marcela Macháčková NRL (National Reference Laboratory) for disinfection and sterilization

MUDr. V. Melicherčíková, CSc.

Center for Epidemiology and Microbiology

MUDr. Barbora Macková

 

 

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Characteristics of the submitted samples

Testimonial requested by: Retap spol. s r.o.
Vaňkova 730, 463 65 Nové Město pod Smrkem

Handled by: Miloš Heršálek (tel.: 603 260 891)

Manufacturer: Retap spol. s r.o. Vaňkova 730, 463 65 Nové Město pod Smrkem

Description of samples: (according to the customer's data)

Two samples of materials were supplied. The first material with the designation 1/1 (larger amount of applied TiO2 composite on Al material) and the second material with the designation 1/2 (smaller amount of applied TiO2 composite on Al material).

Presented documentation: Not provided.

The subject of the expertise was the interpretation of the results of the surface efficiency of the materials of the submitted samples in laboratory experiments.

Interpretation of laboratory test results:

The tested sample of the material marked "1/1" under the action of UV radiation (UV lamp was supplied by the client) showed an anti-virus effect at 3 hours and a virucidal effect at 5 hours, at a temperature of 20-25 ° C and higher relative humidity than 90%.

Virucidal efficacy was tested on a model virus: E. coli bacteriophagus 0X 174.

The tested sample of material marked "1/2" under UV radiation (UV lamp was supplied by the client) showed an anti-virus effect (decrease in pfu increase of 1 log order and more compared to the control) at exposure times of 3 and 5 hours, at a temperature of 20 - 25 ° C and relative humidity higher than 90%. Virucidal efficacy (decrease in pfu increase of 4 log orders of magnitude compared to control) was not demonstrated after 5 hours of treatment.

Virucidal efficacy was tested on a model virus: E. coli bacteriophagus ‹fiX 174.

The samples were not assessed for cleaning properties, corrosivity, toxicity, irritation and occupational safety.

This opinion applies only to the samples submitted and the conclusions drawn from their examination can be applied to other products of the same type only if their composition and properties fully correspond to the samples examined. The test results relate only to the samples listed in the protocol and do not replace others documents. Protocol can be reproduced in its entirety and only with the laboratory’s permission.

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Determination of TiO2 composite efficiency applied to Al material on the liquidation of the virus and irradiation of the UV lamp according to ISO 22196 and JIS Z 2801 methods modified to bacteriophagus

1. Modified method for testing the effectiveness against viruses (bacteriophagus) of non-porous materials according to "Quantitative method for determining the antibacterial activity of plastic (artificial) and other non-porous surfaces (materials) contact test method according to ISO 22196:2011(1).
2. Modified method for testing the effectiveness against viruses (bacteriophagus) of non-porous materials according to "Quantitative method for determining the antibacterial activity of plastic (artificial) and other non-porous surfaces (materials) contact test method according to JIS Z 2801:2010(2).

 

The methods used are not accredited according to the CSN EN ISO/IEC 17025:2018.

Results – nonaccredited methods See Attachment (tables Nr. – 1- 2 )

Tested sample of material marked “1/1” under UV radiation (UV lamp was supplied by the client) showed an anti-virus effect at exposure times of 3 hours and a virucidal effect at 5 hours, at a temperature of 20-25 ° C and higher relative humidity than 90%.

The pfu test showed a reduced account by one log order, confirming the anti-virus effect of the material.

The pfu test showed a reduced account by four log orders, which ensures a virucidal effect.

Virucidal efficacy was tested on a model virus: E. coli bacteriophagus ȹX 174.

The tested sample of material marked "1/2" under UV radiation (UV lamp was supplied by the client) showed an anti-virus effect at exposure times of 3 and 5 hours, at temperature of 20-25 °C and relative humidity higher than 90%. Virucidal efficacy was not demonstrated even after 5 hours of treatment.

The pfu test showed a reduced account by one log order, confirming the anti-virus effect of the material.

The pfu test did not show a reduced account by four log orders, which ensures a virucidal effect.

Virucidal efficacy was tested on a model virus: E. coli bacteriophagus ȹX 174.